香菇多糖与IL-2合用对肺癌浸润淋巴细胞抗瘤活性的影响
作者：李广宙 康白 张磊
单位：李广宙 张磊(潍坊医学院附院核医学科 潍坊 261031)；康白(药理学教研室)
关键词：香菇多糖；IL-2；肿瘤浸润淋巴细胞；抗瘤活性
潍坊医学院000111摘 要：目的探讨香菇多糖与IL-2合用对肿瘤浸润淋巴细胞(TIL)抗瘤活性的影响。方法将TIL分别置于含IL-2或IL-2加香菇多糖培养液中培养30d，观察TIL的扩增能力、抗瘤活性的变化。结果IL-2加香菇多糖培养的Til平均扩增倍数和抗瘤活性均明显高于IL-2培养的TIL。IL-2加香菇多糖培养的TIL对自体瘤细胞，K562和Raji细胞的最大杀伤活性分别为：67.18±14.73%，71.25±15.86%和66.15±15.07%；而单纯IL-2培养的TIL对各种靶细胞的最大杀伤活性分别为43.81±12.17%，50.83±13.56%和45.41±13.76%。两组相比，差异显著(P<0.05)。结论香菇多糖与IL-2合用具有促进TIL扩增，增强其抗瘤活性的作用。
分类号：R734.2；R730.3 文献标识码：A
Influence of Lentinan Combined with IL-2 on Antitumor Activity of Tumor Infiltrating Lymphocytes
Li Guangzhou ,Kang Bai ,Zhang Lei
(Department of Nuclear Medicine，Affiliated Hospital of Weifang Medical College，Weifang，261031)
Abstract：Objective To explore the influence of Lentinan combined with IL-2 on antitumor activity of tumor infiltrating lymphocytes.Method TIL was cultured in IL-2 or IL-2 combined with Lentinan for 30 days，and was compared for proliferation、antitumor activity and phenotypes.Result The mean expansion fold and antitumor activity of TIL cultured in IL-2 combined with Lentinan were higher than that of TIL cultured in I1-2(P<0.05).The biggest cytolytic activity of TIL caltared in IL-2 combined with Lentinan to autologus tumer cells.K562 and Raji cells was 67.18±14.73%，71.25±15.86%and 66.15±15.07%，respectively,and TIL cultured in IL-2 was 43.81±12.17%，50.83±13.56%and 45.41±13.76%，respectively.The cytolytic activety of TIL in two groups was obviously different(P<0.05).Conclusion Lentinan had the effect of promoting the proliferation and antitumor activity of TIL.
Keywords：Lentinan;IL-2;Tumor infiltrating lymphocytes;Antitumor activity
基金项目：山东省卫生厅科研基金资助(批准号95226)
参考文献：
[1]杜泽英，刘锡均，王顺友.香菇多糖对人淋巴细胞IL-2分泌及IL-2R表达的双向调节作用[J].中国实验临床免疫学杂志，1994，6(1)：40
[2]吴昌平，华静，吴爱珍，等.香菇多糖对恶性肿瘤的治疗作用[J].新药与临床，1996，15(1)：29
[3]Rosenberg SA.A new approach to the adoptivc immunotherapy of cancer with TILs[J].Science,1986,233:1318
[4]曹雪涛编著.白细胞介素2的基础与临床[M].北京：科学技术出版社，1990：183～184
收稿日期：1999-12-12, http://www.100md.com
单位：李广宙 张磊(潍坊医学院附院核医学科 潍坊 261031)；康白(药理学教研室)
关键词：香菇多糖；IL-2；肿瘤浸润淋巴细胞；抗瘤活性
潍坊医学院000111摘 要：目的探讨香菇多糖与IL-2合用对肿瘤浸润淋巴细胞(TIL)抗瘤活性的影响。方法将TIL分别置于含IL-2或IL-2加香菇多糖培养液中培养30d，观察TIL的扩增能力、抗瘤活性的变化。结果IL-2加香菇多糖培养的Til平均扩增倍数和抗瘤活性均明显高于IL-2培养的TIL。IL-2加香菇多糖培养的TIL对自体瘤细胞，K562和Raji细胞的最大杀伤活性分别为：67.18±14.73%，71.25±15.86%和66.15±15.07%；而单纯IL-2培养的TIL对各种靶细胞的最大杀伤活性分别为43.81±12.17%，50.83±13.56%和45.41±13.76%。两组相比，差异显著(P<0.05)。结论香菇多糖与IL-2合用具有促进TIL扩增，增强其抗瘤活性的作用。
分类号：R734.2；R730.3 文献标识码：A
Influence of Lentinan Combined with IL-2 on Antitumor Activity of Tumor Infiltrating Lymphocytes
Li Guangzhou ,Kang Bai ,Zhang Lei
(Department of Nuclear Medicine，Affiliated Hospital of Weifang Medical College，Weifang，261031)
Abstract：Objective To explore the influence of Lentinan combined with IL-2 on antitumor activity of tumor infiltrating lymphocytes.Method TIL was cultured in IL-2 or IL-2 combined with Lentinan for 30 days，and was compared for proliferation、antitumor activity and phenotypes.Result The mean expansion fold and antitumor activity of TIL cultured in IL-2 combined with Lentinan were higher than that of TIL cultured in I1-2(P<0.05).The biggest cytolytic activity of TIL caltared in IL-2 combined with Lentinan to autologus tumer cells.K562 and Raji cells was 67.18±14.73%，71.25±15.86%and 66.15±15.07%，respectively,and TIL cultured in IL-2 was 43.81±12.17%，50.83±13.56%and 45.41±13.76%，respectively.The cytolytic activety of TIL in two groups was obviously different(P<0.05).Conclusion Lentinan had the effect of promoting the proliferation and antitumor activity of TIL.
Keywords：Lentinan;IL-2;Tumor infiltrating lymphocytes;Antitumor activity
基金项目：山东省卫生厅科研基金资助(批准号95226)
参考文献：
[1]杜泽英，刘锡均，王顺友.香菇多糖对人淋巴细胞IL-2分泌及IL-2R表达的双向调节作用[J].中国实验临床免疫学杂志，1994，6(1)：40
[2]吴昌平，华静，吴爱珍，等.香菇多糖对恶性肿瘤的治疗作用[J].新药与临床，1996，15(1)：29
[3]Rosenberg SA.A new approach to the adoptivc immunotherapy of cancer with TILs[J].Science,1986,233:1318
[4]曹雪涛编著.白细胞介素2的基础与临床[M].北京：科学技术出版社，1990：183～184
收稿日期：1999-12-12, http://www.100md.com